Compositional Statistical Framework for RNA Fractionation Analysis
beta_binomial_wald: Beta-Binomial Wald Test
DiffPropTest: Statistical Testing for Differential Proportions
extract_condition_matrix: Extract and Prepare Data for Statistical Tes...
FracFixR: Fraction Correction Framework for RNA-seq Data
FracFixR: Main Function for Fraction Correction
logit_diff_test: Logit-based Differential Test
PlotComparison: Create Volcano Plot for Differential Results
PlotFractions: Visualize Fraction Proportions
ProcessReplicate: Core NNLS Regression for Individual Replicates
run_glm: Binomial GLM for Single Transcript
A compositional statistical framework for absolute proportion estimation between fractions in RNA sequencing data. 'FracFixR' addresses the fundamental challenge in fractionated RNA-seq experiments where library preparation and sequencing depth obscure the original proportions of RNA fractions. It reconstructs original fraction proportions using non-negative linear regression, estimates the "lost" unrecoverable fraction, corrects individual transcript frequencies, and performs differential proportion testing between conditions. Supports any RNA fractionation protocol including polysome profiling, sub-cellular localization, and RNA-protein complex isolation.