SPUTNIK1.4.3 package

Spatially Automatic Denoising for Imaging Mass Spectrometry Toolkit

applyPeaksFilter-msi.dataset-method

Apply the results of a peaks filter.

binKmeans-msi.dataset-method

Return a binary mask generated applying k-means clustering on first 10...

binKmeans2-msi.dataset-method

Return a binary mask generated applying k-means clustering on peaks in...

binOtsu-ms.image-method

Binarize MS image using Otsu's thresholding.

binSupervised-msi.dataset-method

Return a binary mask generated applying a supervised classifier on pea...

bladderMALDIRompp2010

Load the example MALDI-MSI data.

closeImage-ms.image-method

Apply morphological closing to binary image.

countPixelsFilter

Filter based on the minimum number of connected pixels in the ROI.

createPeaksFilter

Generate a peak filter object.

CSRPeaksFilter

Performs the peak selection based on complete spatial randomness test.

getIntensityMat-msi.dataset-method

Return the peaks intensity matrix.

getMZ-msi.dataset-method

Return the m/z vector.

getShapeMSI-msi.dataset-method

Returns the geometrical shape of MSI dataset

gini.index

Gini index.

globalPeaksFilter

Reference similarity based peak selection.

invertImage-ms.image-method

Invert the colors of an MS image.

ms.image-class

ms.image-class definition.

msi.dataset-class

msi.dataset-class S4 class definition containing the information about...

msiDataset

Constructor for msi.dataset-class objects.

msImage

Constructor for ms.image-class objects.

NMI

Normalized mutual information (NMI).

normIntensity-msi.dataset-method

Normalize the peaks intensities.

numDetectedMSI-msi.dataset-method

Generates an msImage representing the number of detected peaks per pix...

ovarianDESIDoria2016

Load the example DESI-MSI data.

PCAImage-msi.dataset-method

Generates an RGB msImage representing the first 3 principal components...

plot

Visualize an MS image. plot extends the generic function to ms.image...

refImageBinaryKmeans

Calculate the binary reference image using k-means clustering. K-Means...

refImageBinaryKmeansMulti

Calculate the binary reference image using k-means clustering with mul...

refImageBinaryOtsu

Calculate the binary reference image using Otsu's thresholding.

refImageBinarySVM

Calculate the binary reference image using linear SVM trained on manua...

refImageContinuous

refImageContinuous returns the reference image, calculated using the...

removeSmallObjects-ms.image-method

Remove binary ROI objects smaller than user-defined number of pixels

scatter.ratio

Pixel scatteredness ratio.

smoothImage-ms.image-method

Apply Gaussian smoothing to an MS image.

spatial.chaos

Spatial chaos measure.

splitPeaksFilter

Test for the presence of split peaks.

SSIM

Structural similarity index (SSIM).

totalIonCountMSI-msi.dataset-method

Generates an msImage representing pixels total-ion-counts. This image ...

varTransform-msi.dataset-method

Variance stabilizing transformation.

Set of tools for peak filtering of mass spectrometry imaging data based on spatial distribution of signal. Given a region-of-interest, representing the spatial region where the informative signal is expected to be localized, a series of filters determine which peak signals are characterized by an implausible spatial distribution. The filters reduce the dataset dimension and increase its information vs noise ratio, improving the quality of the unsupervised analysis results, reducing data dimension and simplifying the chemical interpretation. The methods are described in Inglese P. et al (2019) <doi:10.1093/bioinformatics/bty622>.

  • Maintainer: Paolo Inglese
  • License: GPL (>= 3)
  • Last published: 2025-06-23