inder calculates numeric derivatives on smoothed data which results in data points not observable in reality. The rounder function averages such result to the real values of cycle number.
rounder(object, cyc =1)
Arguments
object: a class der object.
cyc: a column containing cycle numbers after smoothing. In case of objects created by the inder function, it is the first column.
Returns
An object of the class der containing cycle number and averaged values of the fluorescence, first derivative and second derivative.