Easy Michaelis-Menten Enzyme Kinetics
ezmmek_calc_mm_fit
ezmmek_calc_std_lm_homo
ezmmek_calc_std_lm_homo
ezmmek_calc_std_lm_homo_buffer
ezmmek_calibrate_activities
ezmmek_std_lm
new_ezmmek_act_calibrate
new_ezmmek_act_group
new_ezmmek_sat_fit
new_ezmmek_std_group
plot_new_ezmmek_act_group
plot_new_ezmmek_calibrate
plot_new_ezmmek_sat_fit
plot_new_ezmmek_std_group
Serves as a platform for published fluorometric enzyme assay protocols. 'ezmmek' calibrates, calculates, and plots enzyme activities as they relate to the transformation of synthetic substrates. At present, 'ezmmek' implements two common protocols found in the literature, and is modular to accommodate additional protocols. Here, these protocols are referred to as the In-Sample Calibration (Hoppe, 1983; <doi:10.3354/meps011299>) and In-Buffer Calibration (German et al., 2011; <doi:10.1016/j.soilbio.2011.03.017>). protocols. By containing multiple protocols, 'ezmmek' aims to stimulate discussion about how to best optimize fluorometric enzyme assays. A standardized approach would make studies more comparable and reproducible.