Analyze MS/MS Protein Melting Data
Convert absolute quantitation to relative ratios.
MSResultSet to data frame.
Generate protein ratio profile from spectrum quantification matrix.
Model MSThermExperiment.
Model single protein.
Model and analyze MS/MS-based protein melting data.
Create a new MSThermExperiment.
Normalize to a profile.
Normalize to a spike-in standard.
Re-normalize based on Tm.
Plot MSThermResult object.
Plot MSThermResultSet object.
Summarize MSThermResult object.
Summarize MSThermResultSet object.
Export MSThermResultSet to an SQLite database.
Software to aid in modeling and analyzing mass-spectrometry-based proteome melting data. Quantitative data is imported and normalized and thermal behavior is modeled at the protein level. Methods exist for normalization, modeling, visualization, and export of results. For a general introduction to MS-based thermal profiling, see Savitski et al. (2014) <doi:10.1126/science.1255784>.