extract_depth function

Extract allele counts of progeny and parents of vcf file

Uses vcfR package and onemap object to generates list of vectors with reference allele count and total counts for each marker and genotypes included in onemap object (only available for biallelic sites)

extract_depth(
  vcfR.object = NULL,
  onemap.object = NULL,
  vcf.par = c("GQ", "AD", "DPR, PL", "GL"),
  parent1 = "P1",
  parent2 = "P2",
  f1 = "F1",
  recovering = FALSE
)

Arguments

• vcfR.object: object output from vcfR package
• onemap.object: onemap object output from read_onemap, read_mapmaker or onemap_read_vcf function
• vcf.par: vcf format field that contain allele counts informations, the implemented are: AD, DPR, GQ, PL, GL. AD and DPR return a list with allele depth information. GQ returns a matrix with error probability for each genotype. PL return a data.frame with genotypes probabilities for every genotype.
• parent1: parent 1 identification in vcfR object
• parent2: parent 2 identification in vcfR object
• f1: if your cross type is f2, you must define the F1 individual
• recovering: TRUE/FALSE, if TRUE evaluate all markers from vcf file, if FALSE evaluate only markers in onemap object

Returns

list containing the following components: - palt: a matrix with parent 1 and 2 alternative allele counts. - pref: a matrix with parent 1 and 2 reference allele counts. - psize: a matrix with parent 1 and 2 total allele counts.- oalt: a matrix with progeny alternative allele counts.- oref: a matrix with progeny reference allele counts.- osize: a matrix with progeny total allele counts.- n.mks: total number of markers.

• n.ind: total number of individuals in progeny. - inds: progeny individuals identification.

• mks: markers identification. - onemap.object: same onemap.object inputted

Author(s)

Cristiane Taniguti, chtaniguti@tamu.edu