Analyzing Real-Time Quantitative PCR Data
Calculate the amounts
Get vector average by a variable
Get vector coefficient of variance by a variable
Propage two vectors
Calculate the intercept of a line
Normalize vector by another
Plotting function
Plot quality assessment graphs
Raise two to a vector power
Calculate R squared
Get vector standard deviation by a variable
Calculate the slope of a line
pcr package
Apply qPCR analysis methods
Assess qPCR data quality
Calculate the standard curve model
Calculate the delta_ct model
Calculate the delta_delta_ct model
Calculate amplification efficiency
Linear regression qPCR data
Calculate the standard curve
Statistical testing of PCR data
t-test qPCR data
Wilcoxon test qPCR data
Calculates the amplification efficiency and curves from real-time quantitative PCR (Polymerase Chain Reaction) data. Estimates the relative expression from PCR data using the double delta CT and the standard curve methods Livak & Schmittgen (2001) <doi:10.1006/meth.2001.1262>. Tests for statistical significance using two-group tests and linear regression Yuan et al. (2006) <doi: 10.1186/1471-2105-7-85>.