qpcR1.4-1 package

Modelling and Analysis of Real-Time PCR Data

AICc

Akaike's second-order corrected Information Criterion

akaike.weights

Calculation of Akaike weights/relative likelihoods/delta-AICs

calib.rd

Calculation of qPCR efficiency using dilution curves and replicate boo...

Cy0.rd

Cy0 alternative to threshold cycles as in Guescini et al. (2008)

eff

The amplification efficiency curve of a fitted object

efficiency

Calculation of qPCR efficiency and other important qPCR parameters

evidence

Evidence ratio for model comparisons with AIC, AICc or BIC

expcomp

Comparison of all sigmodal models within the exponential region

expfit

Calculation of PCR efficiency by fitting an exponential model

fitchisq.rd

The chi-square goodness-of-fit

getPar.rd

Batch calculation of qPCR fit parameters/efficiencies/threshold cycles...

is.outlier.rd

Outlier summary for objects of class 'modlist' or 'replist'

KOD.rd

(K)inetic (O)utlier (D)etection using several methods

llratio.rd

Calculation of likelihood ratios for nested models

LOF.test.rd

Formal lack-Of-Fit test of a nonlinear model against a one-way ANOVA m...

LRE

Calculation of qPCR efficiency by the 'linear regression of efficiency...

maxRatio.rd

The maxRatio method as in Shain et al. (2008)

meltcurve.rd

Melting curve analysis with (iterative) Tm identification and peak are...

midpoint.rd

Calculation of the 'midpoint' region

modlist

Create nonlinear models from a dataframe and coerce them into a list

mselect

Sigmoidal model selection by different criteria

parKOD.rd

Parameters that can be changed to tweak the kinetic outlier methods

pcrbatch

Batch calculation of qPCR efficiency and other qPCR parameters

pcrboot.rd

Bootstrapping and jackknifing qPCR data

pcrfit

Workhorse function for qPCR model fitting

pcrGOF

Summarize measures for the goodness-of-fit

pcrimport

Advanced qPCR data import function

pcrimport2

Simple qPCR data import function (i.e. from text files or clipboard)

pcropt1

Combinatorial elimination of plateau and ground phase cycles

pcrsim.rd

Simulation of sigmoidal qPCR data with goodness-of-fit analysis

plot.pcrfit

Plotting qPCR data with fitted curves

predict.pcrfit.rd

Value prediction from a fitted qPCR model

PRESS

Allen's PRESS (Prediction Sum-Of-Squares) statistic, aka P-square

propagate

Error propagation using different methods

qpcR.news.rd

Display news and changes of qpcR package versions

qpcR_datasets.rd

The (published) datasets implemented in qpcR

qpcR_functions.rd

The nonlinear/mechanistic models implemented in qpcR

ratiobatch.rd

Calculation of ratios in a batch format for multiple genes/samples

ratiocalc

Calculation of ratios from qPCR runs with/without reference genes

ratioPar.rd

Calculation of ratios in a batch format from external PCR parameters

refmean

Averaging of multiple reference genes

replist.rd

Amalgamation of single data models into a model containing replicates

resplot

An (overlayed) residuals barplot

resVar

Residual variance of a fitted model

RMSE

Root-mean-squared-error of a fitted model

Rsq.ad.rd

Adjusted R-square value of a fitted model

Rsq

R-square value of a fitted model

RSS

Residual sum-of-squares of a fitted model

sliwin

Calculation of qPCR efficiency by the 'window-of-linearity' method

takeoff.rd

Calculation of the qPCR takeoff point

update.pcrfit.rd

Updating and refitting a qPCR model

Model fitting, optimal model selection and calculation of various features that are essential in the analysis of quantitative real-time polymerase chain reaction (qPCR).

  • Maintainer: Andrej-Nikolai Spiess
  • License: GPL (>= 2)
  • Last published: 2018-06-14