qPCR Data Analysis
Delta Ct ANOVA analysis with optional model specification
Delta Delta Ct ANOVA analysis with optional model specification
Cleaning data and weighted delta Ct (wDCt) calculation
Amplification efficiency statistics and standard curves
Internal global variables
Converts a 4-column qPCR long data format to wide format
Delta Delta Ct pairwise comparisons using a fitted model
Computing the mean of technical replicates
Combine multiple ggplot objects into a single layout
Bar plot of gene expression for 1-, 2-, or 3-factor experiments
Delta Delta Ct method t-test analysis
Delta Delta Ct method wilcox.test analysis
Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-test, wilcox.test, ANOVA models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.