Metabolic State Space and Trajectory Analysis for Single-Cell Data
Calculate metabolic pathway scores for single cells
Identify metabolic trajectory switchpoint
Identify candidate transition zone along mPT
Compute module trends along mPT stratified by metabolic cluster
Compute trends and switchpoints for multiple modules along mPT
Compute metabolic module trend along pseudotime
Calculate metabolic axis scores
Summarize metabolic profiles of clusters
Cluster cells in metabolic PCA space
Embed cells in metabolic feature space
Compute local directional consistency along mPT
Prepare mPT distribution data for metabolic subclusters
Plot metabolic gradient on UMAP
Plot metabolic module cards
Plot module trends by metabolic cluster
Plot multiple module trends with switchpoints
Provides a framework for modeling cellular metabolic states and continuous metabolic trajectories from single-cell RNA-seq data using pathway-level scoring. Enables lineage-restricted metabolic analysis, metabolic 'pseudotime' inference, module-level trend analysis, and visualization of metabolic state transitions.