Small Molecule Octet/BLI Kinetics Experiment
~~ Methods for Function alignLoad ~~
Align BLI traces with loading step
~~ Methods for Function baseline ~~
Align BLI traces with baseline step
Class "Bli"
Constructor of "Bli" class
BLI data example
~~ Methods for Function doubleBlank ~~
Substract the double-references
~~ Methods for Function estimate ~~
Estimate initial kinetic parameters
~~ Methods for Function fitKinetics ~~
Fit binding kinetics
~~ Methods for Function initialize ~~
~~ Methods for Function kinetics ~~
Output kinetics model
~~ Methods for Function kOff0 ~~
Getter for "Bli" slot of kOff0
~~ Methods for Function kOff0<- ~~
Setter for "Bli" slot of kOff0
~~ Methods for Function kOn0 ~~
Getter for "Bli" slot of kOn0
~~ Methods for Function kOn0<- ~~
Setter for "Bli" slot of kOn0
~~ Methods for Function ligand ~~
Getter for "Bli" slot of ligand concentration
~~ Methods for Function ligand<- ~~
Setter for "Bli" slot of ligand concentration
Class nls
~~ Methods for Function plotKinetics ~~
Plot kinetics model fitting
~~ Methods for Function plotResiduals ~~
Plot residuals from kinetics model fitting
~~ Methods for Function plotTraces ~~
Plot BLI traces
~~ Methods for Function show ~~
tools:::Rd_package_title("smoke")
~~ Methods for Function status ~~
Getter for data processing status
~~ Methods for Function tExp ~~
Getter for BLI times tExp
~~ Methods for Function tExp<- ~~
Setter for BLI times tExp
~~ Methods for Function traces ~~
Getter of BLI traces
~~ Methods for Function traces<- ~~
Setter of BLI traces
Bio-Layer Interferometry (BLI) is a technology to determine the binding kinetics between biomolecules. BLI signals are small and noisy when small molecules are investigated as ligands (analytes). We develop this package to process and analyze the BLI data acquired on Octet Red96 from Fortebio more accurately. Sun Q., Li X., et al (2020) <doi:10.1038/s41467-019-14238-3>. In this new version, we organize the BLI experiment data and analysis methods into a S4 class with self-explaining structure.