HaDeX21.0.0 package

Analysis and Visualisation of Hydrogen/Deuterium Exchange Mass Spectrometry Data

add_stat_dependency

Calculates confidence limits

calculate_aggregated_diff_uptake

Calculates aggregated deuterium uptake difference for one time point

calculate_aggregated_test_results

Aggregate test result

calculate_aggregated_uptake

Calculates aggregated deuterium uptake for one time point

calculate_auc

Calculate Area Under the Curve

calculate_back_exchange

Back exchange estimation

calculate_confidence_limit_values

Calculate the value of confidence limit

calculate_diff_uptake

Calculate differential uptake

calculate_exp_masses_per_replicate

Calculate measured mass for each replicate of the experiment

calculate_exp_masses

Calculate measured mass, aggregated from the replicates of the experim...

calculate_kinetics

Calculate kinetics data

calculate_MHP

Calculate MHP of the peptide

calculate_p_value

Create p-value dataset

calculate_peptide_kinetics

Calculate kinetics dataset

calculate_state_uptake

Calculate deuterium uptake

create_aggregated_diff_uptake_dataset

Calculates aggregated uptake difference for peptide pool

create_aggregated_uptake_dataset

Calculates the aggregated uptake for peptide pool

create_control_dataset

Create dataset with control

create_diff_uptake_dataset

Generate differential dataset

create_kinetic_dataset

Create kinetics dataset for a list of peptides and their states

create_overlap_distribution_dataset

Show overlap distribution data

create_p_diff_uptake_dataset_with_confidence

Create differential dataset with statistical validity

create_p_diff_uptake_dataset

Create differential uptake dataset with p-value

create_quality_control_dataset

Experiment quality control

create_replicate_dataset

Create replicates data

create_state_comparison_dataset

Creates comparison uptake dataset

create_state_uptake_dataset

Create uptake dataset for chosen state

create_uptake_dataset

Create uptake dataset for multiple states

get_n_replicates

Get number of replicates

get_peptide_sequence

Get peptide sequence based on the position

get_protein_coverage

Get protein coverage

get_protein_redundancy

Get protein redundancy

get_replicate_list_sd

Get replicates sd

get_residue_positions

Get residue positions

get_structure_color

Get color palette for 3D structure

HaDeX_GUI

HaDeX Graphical User Interface

HaDeX2-package

HaDeX2

HaDeXify

HaDeX customized ggplot theme

hdx_data

Creation of validated hdx_data class

install_GUI

Installs GUI package from GitHub

is_GUI_installed

Checks if GUI package is installed

new_hdx_data

Constructor of hdx_data class

plot_aggregated_differential_uptake

Plots aggregated uptake difference

plot_aggregated_uptake_structure

Plot aggregated uptake on the 3D structure

plot_aggregated_uptake

Plots aggregated uptake

plot_amino_distribution

generate_amino_distribution

plot_butterfly

Butterfly deuterium uptake plot

plot_chiclet

Chiclet deuterium uptake plot

plot_coverage_heatmap

Coverage heatmap

plot_coverage

Peptide coverage

plot_differential_butterfly

Butterfly differential deuterium uptake plot

plot_differential_chiclet

Chiclet differential deuterium uptake plot

plot_differential_uptake_curve

Plot differential uptake curve

plot_differential

Differential plot

plot_manhattan

Manhattan plot

plot_overlap_distribution

Plot overlap distribution

plot_overlap

Plot overlap data

plot_peptide_charge_measurement

Plot peptide charge measurement

plot_peptide_mass_measurement

Plot peptide mass measurement

plot_position_frequency

Position frequency

plot_quality_control

Plot quality control data

plot_replicate_histogram

Plot replicates histogram

plot_replicate_mass_uptake

Replicate mass uptake curve

plot_state_comparison

State deuterium uptake comparison

plot_uncertainty

Uncertainty of the peptide measurements

plot_uptake_curve

Deuterium uptake curve

plot_volcano

Volcano plot

prepare_hdxviewer_export

Prepares data export for HDX-Viewer

read_hdx

Read HDX-MS data file

reconstruct_sequence

Reconstruct protein sequence

show_aggregated_uptake_data

Show aggregated values in friendly form

show_coverage_heatmap_data

Coverage heatmap data

show_diff_uptake_data_confidence

Differential uptake data with confidence

show_diff_uptake_data

Differential deuterium uptake data

show_overlap_data

Show data on peptide overlap

show_p_diff_uptake_data

Differential deuterium uptake data

show_peptide_charge_measurement

Show peptide charge measurement

show_peptide_mass_measurement

Show peptide mass measurement

show_quality_control_data

Show quality control data

show_replicate_histogram_data

Show replicate data

show_summary_data

Summary data

show_uc_data

Deuterium uptake curve data

show_uptake_data

Deuterium uptake data

update_hdexaminer_file

Update HDeXaminer datafile

validate_hdx_data

Validator on hdx_data class

Processing, analysis and visualization of Hydrogen Deuterium eXchange monitored by Mass Spectrometry experiments (HDX-MS). 'HaDeX2' introduces a new standardized and reproducible workflow for the analysis of the HDX-MS data, including uncertainty propagation, data aggregation and visualization on 3D structure. Additionally, it covers data exploration, quality control and generation of publication-quality figures. All functionalities are also available in the accompanying 'shiny' app.

  • Maintainer: Weronika Puchala
  • License: GPL-3
  • Last published: 2026-02-09 13:30:26 UTC