conos1.5.2 package

Clustering on Network of Samples

armaCor

A slightly faster way of calculating column correlation matrix

basicSeuratProc

Create and preprocess a Seurat object

bestClusterThresholds

Find threshold of cluster detectability

bestClusterTreeThresholds

Find threshold of cluster detectability in trees of clusters

buildWijMatrix

Rescale the weights in an edge matrix to match a given perplexity.

checkCountsWholeNumbers

Check that the count data contain only integer counts

Conos

Conos R6 class

convertToPagoda2

Convert Conos object to Pagoda2 object

edgeMat

Set edge matrix edgeMat with certain values on sample

estimateWeightEntropyPerCell

Estimate entropy of edge weights per cell according to the specified f...

filter.genes.by.cluster.expression

Filter genes by requiring minimum average expression within at least o...

findSubcommunities

Increase resolution for a specific set of clusters

getBetweenCellTypeCorrectedDE

Compare two cell types across the entire panel

getBetweenCellTypeDE

Compare two cell types across the entire panel

getCellNames

Access cell names from sample

getClustering

Access clustering from sample

getClusteringGroups

Extract specified clustering from list of conos clusterings

getClusterRelationshipConsistency

Evaluate consistency of cluster relationships Using the clustering we ...

getCountMatrix

Access count matrix from sample

getEmbedding

Access embedding from sample

getGeneExpression

Access gene expression from sample

getGenes

Access genes from sample

getGlobalClusterMarkers

Deprecated; Get markers for global clusters

getNeighborMatrix

Establish rough neighbor matching between samples given their projecti...

getOdGenesUniformly

Get top overdispersed genes across samples

getOverdispersedGenes

Access overdispersed genes from sample

getPca

Access PCA from sample

getPerCellTypeDE

Do differential expression for each cell type in a conos object betwee...

getPercentGlobalClusters

Evaluate how many clusters are global

getRawCountMatrix

Access raw count matrix from sample

getSampleNamePerCell

Retrieve sample names per cell

greedyModularityCut

Performs a greedy top-down selective cut to optmize modularity

multimulti.community

Constructrs a two-step clustering, first running multilevel.communitie...

multitrap.community

Constructs a two-step clustering, first running multilevel.communities...

namedLevels

Get a vector with the levels of a factor named with their own name. Us...

namedNames

Get a vector of the names of an object named by the names themselves. ...

p2app4conos

Utility function to generate a pagoda2 app from a conos object

plotClusterBarplots

Plots barplots per sample of composition of each pagoda2 application b...

plotClusterBoxPlotsByAppType

Generate boxplot per cluster of the proportion of cells in each cellty...

plotComponentVariance

Plot fraction of variance explained by the successive reduced space co...

plotDEheatmap

Plot a heatmap of differential genes

plotEmbeddings

Plot panel of specified embeddings

plotSamples

Plot panel of specified embeddings, extracting them from pagoda2 objec...

projectKNNs

Project a distance matrix into a lower-dimensional space.

propagateLabelsDiffusion

Estimate labeling distribution for each vertex, based on provided labe...

quickCCA

Perform CCA (using PMA package or otherwise) on two samples

quickCPCA

Perform cpca on two samples

quickPlainPCA

Use space of combined sample-specific PCAs as a space

rawMatricesWithCommonGenes

Get raw matrices with common genes

reexports

Objects exported from other packages

saveConosForScanPy

Save Conos object on disk to read it from ScanPy

saveDEasCSV

Save differential expression as table in *csv format

saveDEasJSON

Save differential expression results as JSON

scanKModularity

Scan joint graph modularity for a range of k (or k.self) values Builds...

sgdBatches

Calculate the default number of batches for a given number of vertices...

stableTreeClusters

Determine number of detectable clusters given a reference walktrap and...

velocityInfoConos

RNA velocity analysis on samples integrated with conos Create a list o...

Wires together large collections of single-cell RNA-seq datasets, which allows for both the identification of recurrent cell clusters and the propagation of information between datasets in multi-sample or atlas-scale collections. 'Conos' focuses on the uniform mapping of homologous cell types across heterogeneous sample collections. For instance, users could investigate a collection of dozens of peripheral blood samples from cancer patients combined with dozens of controls, which perhaps includes samples of a related tissue such as lymph nodes. This package interacts with data available through the 'conosPanel' package, which is available in a 'drat' repository. To access this data package, see the instructions at <https://github.com/kharchenkolab/conos>. The size of the 'conosPanel' package is approximately 12 MB.

  • Maintainer: Evan Biederstedt
  • License: GPL-3
  • Last published: 2024-02-26