Processing and Analyzing Amplicon Sequence Data
Combine FASTA/FASTQ files in a directory into a single file or object
Synchronize FASTA and FASTQ files or objects
Make Sintax database
Convert phyloseq object to Rsearch object
Plot quality scores per position for FASTQ reads
Plot distribution of expected error (EE) rate of reads
Plot read length vs. read quality
Plot distribution of size values
Create Rsearch object
Rsearch
Convert Rsearch object to phyloseq object
Set the VSEARCH executable
Make a taxonomy tree
Taxonomic classification with LCA
Cluster FASTA sequences
Cluster FASTA sequences
Denoising FASTA sequences
Join paired-end sequence reads
Merge paired-end sequence reads
Subsample sequences
Trim and/or filter sequences in FASTA/FASTQ format
Dereplicate sequences
Length statistics after merging
Optimize read truncation with truncee_rate
Optimize read truncation with truncqual
Search for exact full-length matches
Taxonomic classification using the Sintax algorithm
Detect chimeras without external references (i.e. de novo)
Detect chimeras by comparing sequences to a reference database
Global pairwise alignment
Test if VSEARCH can be executed
Processing and analysis of targeted sequencing data. The package provides a user-friendly interface for core 'VSEARCH' (Rognes et al. (2016), <doi:10.7717/peerj.2584>) functions, in addition to tools for visualization and parameter tuning.
Useful links